Crop Genebank Knowledge Base

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Terms used on the Crop Genebank Knowledge Base.

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Term Definition
Pyrimidine

A nitrogen-containing

Pyrophosphate

A chemical molecule that is released each time a deoxynucleotide is incorporated to the new DNA strand during pyrosequencing.

Pyrosequencing

A new sequencing method of relatively short DNA templates based on real-time (quantitative) pyrophosphate release.

Quarantine

The official confinement of introduced germplasm subject to phytosanitary regulations to ensure that it does not carry diseases or pests injurious to the importing country.

Radioactive labeling

The labeling of probes or primers with radioactive tags in order to enable detection of variation in DNA fragments.

Random Amplified Polymorphic DNA

(RAPD) A molecular marker technique for amplifying anonymous stretches of DNA using PCR with arbitrary primers.

Random sample

A sample drawn at random from a larger group.

Re-synthesized/Recreated

In studies of hybrid origins of species, investigators may attempt to ???re-synthesize??? a hybrid by crossing its putative parents and compare the natural to the putative hybrid by morphological or molecular markers.

Recalcitrant seeds

Seeds that loose viability when dried or stored at low temperatures.

Recombination

The production of a DNA molecule with segments derived from more than one parental DNA molecule. In eukaryotes

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Sources for this glossary: IPGRI Tech. Bulletin 10: Molecular markers for genebank management and Seed handling in genebanks training course
Glossary open source software by M. Brampton

International Agricultural Research Centres who worked together to make this site possible:
Africa Rice Center | Bioversity International | CIAT | CIMMYT | CIP | ICARDA | ICRISAT | IFPRI | IITA | ILRI | IRRI |

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