Crop Genebank Knowledge Base

IRRI genebank

IRRI (Rice) genebank operations manual

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This manual describes how and why the various operations and procedures of rice genetic conservation at the International Rice Research Institute (IRRI) are carried out. Since its original compilation in 1995, several modifications have been introduced in the work activities. These are incorporated in this revised edition (2000) of the Manual.

IRRI hopes readers of this Manual will find it informative and useful in their own conservation programs.

Contents

CHAPTER 1 - CONSERVATION OF RICE GENETIC RESOURCES AT IRRI

The conservation of rice genetic resources at IRRI
The need for genetic conservation
The genebank
Germplasm acquisition and conservation
Germplasm exchange
Germplasm characterization and evaluation
Data management

CHAPTER 2 - SEED MULTIPLICATION OF ORYZA SATIVA AND O. GLABERRIMA GERMPLASM

Seed multiplication of Oryza sativa and O. Glaberrima germplasm
Selection of planting materials
Seeding and seedbed activities
Field activities

CHAPTER 3 - SEED CONSERVATION

Introduction to seed conservation
Harvest verification
Seed cleaning and selection
Viability testing
Seed health evaluation
Final drying
Packing
Duplicate storage
Maintenance

CHAPTER 4 - GERMPLASM NURSERY SCREENHOUSES

The germplasm nursery screenhouses
Cultivated species
Wild rices

CHAPTER 5 - GERMPLASM CHARACTERIZATION AND EVALUATION

Introduction to germplasm characterization and evaluation
Selection of Materials for Characterization
Plant Establishment
Morpho-Agronomic Characterization

CHAPTER 6 - GERMPLASM EXCHANGE

Receipt of germplasm
Processing newly received germplasm
Protocol for seed distribution
IRRI’s policy on germplasm exchange
IRRI’s Policy on Intellectual Property Rights
Protocol for requesting information

CHAPTER 7 - DOCUMENTATION AND EXCHANGE OF GERMPLASM INFORMATION

Documentation and exchange of germplasm information
Background
System accessibility
System objectives
System menu structure
Features and capabilities
IRCGIS User's Manual
Data integrity
Data security
Data exchange
System documentation

CHAPTER 8 - MOLECULAR MARKER LABORATORY

Molecular marker laboratory
Protocol for DNA Sample Preparation
RAPD Protocol
Microsatellite Protocol
AFLP Protocol
Abbreviations/Acronyms

CHAPTER 9 - CONSERVATION SUPPORT

Conservation support
In vitro germination
Cytology
Orcein

APPENDICES