Health diagnosis of radish genetic resources
Contributors to this page: CAAS, China (Qiu Yang, Li Xixiang); Bioversity International, Italy (Imke Thormann, Ehsan Dulloo); CGN, Netherlands (Noortje Bas); IPK, Germany (Andreas Börner, Ulrike Lohwasser); AVRDC, Taiwan (Andreas Ebert); USDA, USA (Larry Robertson); NBPGR, India (Chitra Pandey); SASA, UK (George Campbell); University of Warwick, UK (Charlotte Allender).
List of pests and diseases of quarantine importance
The list below mentions some of the diseases that are considered important worldwide, but many of them may or may not have relevance in specific countries. Diseases of limited relevance (e.g. only important in very few countries) are not listed.
Bacteria
- Black Rot (Xanthomonas campestris pv. campestris)
- Bacterial leaf spot, Peppery leaf spot (Pseudomonas syringae pv.maculicola)
Fungi
- Alternaria leaf spot (Alternaria brassicae, A. brassicicola, and A. raphani)
- Black leg (Leptosphaeria maculans (anamorph Phoma lingam))
- Fusarium yellows or Fusarium wilt of radish (Fusarium oxysporum f. sp. raphani)
- Downy mildew (Peronospora parasitica)
- Clubroot (Plasmodiophora brassicae)
- Rhizoctonia scurf (Rhizoctonia solani)
- Sclerotinia (Sclerotinia sclerotiorum)
- White Rust (Albugo candida)
- Black root (Aphanomyces raphani)
Testing procedures
Recommended methods to detect the presence of each disease:
- Seed borne fungi (Blotter test).
- Bacteria (Indicator plant method)
Testing intervals/seasons
- Testing before material goes into the genebank or to the field is important to reduce transfer of diseases or pests.
Recording information during health diagnosis
The following information should be recorded for each health diagnosis step:
- Accession number (ID number).
- Lot number (ID number).
- Date of test.
- Number of replicates.
- Number of seeds sampled and tested per replicate.
- Pre-treatments (pre treatments used for the test).
- Test method.
- Duration of test
- Diseases identified.
- Incidence of each disease (%)
References and further reading
ISTA. 2005. International Rules for Seed Testing. Edition 2005. International Seed Testing Association, Bassersdorf, Switzerland.
Recommended Codes for Pest Organisms in Vegetable Crops. 2009. [online]. Available from: http://www.worldseed.org/cms/medias/file/TradeIssues/PhytosanitaryMatters/PathogenCoding/RecommendedCodesForPestOrganisms/
Path_Codes_2009_NL.pdf. Date accessed: 7 June 2010.
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